American Journal of Epidemiology Advance Access originally published online on July 13, 2005
American Journal of Epidemiology 2005 162(4):373-381; doi:10.1093/aje/kwi213
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
PRACTICE OF EPIDEMIOLOGY |
Fasting Whole Blood as a Biomarker of Essential Fatty Acid Intake in Epidemiologic Studies: Comparison with Adipose Tissue and Plasma
1 Department of Nutrition, Harvard School of Public Health, Boston, MA
2 Hanyang University College of Medicine, Seoul, South Korea
3 Central American Center for Population, University of Costa Rica, San Pedro de Montes de Oca, Costa Rica
Correspondence to Dr. Ana Baylin, Department of Nutrition, Harvard School of Public Health, 665 Huntington Avenue, SPH2, Boston, MA 02115 (e-mail: abaylin{at}hsph.harvard.edu).
Biomarkers could provide a more accurate measure of long-term intake than questionnaires. Adipose tissue is considered the best indicator of long-term essential fatty acid intake, but other tissues may prove equally valid. The authors evaluated the ability of fasting whole blood, relative to fasting plasma and adipose tissue, to reflect fatty acid intake. Costa Rican men (n = 99) and women (n = 101) completed a 135-item food frequency questionnaire and provided adipose tissue and blood samples from 1999 to 2001. Fatty acids were identified by using capillary gas chromatography. Correlation coefficients adjusted for age, sex, and body mass index were calculated. Diet-tissue correlation coefficients for 
-linolenic acid and linoleic acid, respectively, were 0.38 and 0.43 in whole blood, 0.51 and 0.52 in adipose tissue, and 0.39 and 0.41 in plasma. High correlations were observed between whole-blood -linolenic and linoleic acid and adipose tissue (r = 0.59 and r = 0.67) and plasma (r = 0.96 and r = 0.88), respectively. Results show that fasting whole blood is a suitable biomarker of long-term essential fatty acid intake, and its performance is comparable to that of fasting plasma. Thus, fasting whole blood could be the sample of choice in epidemiologic studies because of its ability to predict intake, its accessibility, and minimum sample processing.
adipose tissue; blood; Costa Rica; fasting; fatty acids; plasma
Abbreviations: FFQ, food frequency questionnaire
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Q. Sun, J. Ma, H. Campos, K. M Rexrode, C. M Albert, D. Mozaffarian, and F. B Hu Blood concentrations of individual long-chain n-3 fatty acids and risk of nonfatal myocardial infarction Am. J. Clinical Nutrition, July 1, 2008; 88(1): 216 - 223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Oken, J. S. Radesky, R. O. Wright, D. C. Bellinger, C. J. Amarasiriwardena, K. P. Kleinman, H. Hu, and M. W. Gillman Maternal Fish Intake during Pregnancy, Blood Mercury Levels, and Child Cognition at Age 3 Years in a US Cohort Am. J. Epidemiol., May 15, 2008; 167(10): 1171 - 1181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. N. Hall, J. E. Chavarro, I-M. Lee, W. C. Willett, and J. Ma A 22-year Prospective Study of Fish, n-3 Fatty Acid Intake, and Colorectal Cancer Risk in Men Cancer Epidemiol. Biomarkers Prev., May 1, 2008; 17(5): 1136 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Agostoni, E Riva, M Giovannini, F Pinto, C Colombo, P Rise, C Galli, and F Marangoni Maternal smoking habits are associated with differences in infants' long-chain polyunsaturated fatty acids in whole blood: a case-control study Arch. Dis. Child., May 1, 2008; 93(5): 414 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Chavarro, M. J. Stampfer, H. Campos, T. Kurth, W. C. Willett, and J. Ma A Prospective Study of Trans-Fatty Acid Levels in Blood and Risk of Prostate Cancer Cancer Epidemiol. Biomarkers Prev., January 1, 2008; 17(1): 95 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Sun, J. Ma, H. Campos, and F. B Hu Plasma and erythrocyte biomarkers of dairy fat intake and risk of ischemic heart disease Am. J. Clinical Nutrition, October 1, 2007; 86(4): 929 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cherubini, C. Andres-Lacueva, A. Martin, F. Lauretani, A. D. Iorio, B. Bartali, A. Corsi, S. Bandinelli, M. P. Mattson, and L. Ferrucci Low Plasma N-3 Fatty Acids and Dementia in Older Persons: The InCHIANTI Study J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2007; 62(10): 1120 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Sun, J. Ma, H. Campos, S. E Hankinson, and F. B Hu Comparison between plasma and erythrocyte fatty acid content as biomarkers of fatty acid intake in US women Am. J. Clinical Nutrition, July 1, 2007; 86(1): 74 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Chavarro, M. J. Stampfer, H. Li, H. Campos, T. Kurth, and J. Ma A Prospective Study of Polyunsaturated Fatty Acid Levels in Blood and Prostate Cancer Risk Cancer Epidemiol. Biomarkers Prev., July 1, 2007; 16(7): 1364 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baylin, E. Ruiz-Narvaez, P. Kraft, and H. Campos {alpha}-Linolenic acid, {Delta}6-desaturase gene polymorphism, and the risk of nonfatal myocardial infarction Am. J. Clinical Nutrition, February 1, 2007; 85(2): 554 - 560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Hall, H. Campos, H. Li, H. D. Sesso, M. J. Stampfer, W. C. Willett, and J. Ma Blood Levels of Long-Chain Polyunsaturated Fatty Acids, Aspirin, and the Risk of Colorectal Cancer Cancer Epidemiol. Biomarkers Prev., February 1, 2007; 16(2): 314 - 321. [Abstract] [Full Text] [PDF]
|
|