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Am J Epidemiol 2002; 156:871-881.
Copyright © 2002 by Johns Hopkins Bloomberg School of Public Health


PRACTICE OF EPIDEMIOLOGY

Multi-Ethnic Study of Atherosclerosis: Objectives and Design

Diane E. Bild1, David A. Bluemke2, Gregory L. Burke3, Robert Detrano4, Ana V. Diez Roux5, Aaron R. Folsom6, Philip Greenland7, David R. JacobsJr.6, Richard Kronmal8, Kiang Liu7, Jennifer Clark Nelson8, Daniel O’Leary9, Mohammed F. Saad10, Steven Shea5, Moyses Szklo11 and Russell P. Tracy12

1 Division of Epidemiology and Clinical Applications, National Heart, Lung, and Blood Institute, Bethesda, MD.
2 Department of Radiology and Radiological Science, School of Medicine, Johns Hopkins University, Baltimore, MD.
3 Department of Public Health Sciences, School of Medicine, Wake Forest University, Winston-Salem, NC.
4 Harbor-UCLA Research and Education Institute, Los Angeles, CA.
5 Departments of Medicine and Epidemiology, Schools of Medicine and Public Health, Columbia University, New York, NY.
6 Division of Epidemiology, School of Public Health, University of Minnesota, Minneapolis, MN.
7 Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL.
8 Department of Biostatistics, School of Public Health and Community Medicine, University of Washington, Seattle, WA.
9 Department of Radiology, New England Medical Center, Boston, MA.
10 Department of Medicine, School of Medicine, University of California, Los Angeles, Los Angeles, CA.
11 Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD.
12 Laboratory for Clinical Biochemistry Research, Departments of Pathology and Biochemistry, College of Medicine, University of Vermont, Burlington, VT.

The Multi-Ethnic Study of Atherosclerosis was initiated in July 2000 to investigate the prevalence, correlates, and progression of subclinical cardiovascular disease (CVD) in a population-based sample of 6,500 men and women aged 45–84 years. The cohort will be selected from six US field centers. Approximately 38% of the cohort will be White, 28% African-American, 23% Hispanic, and 11% Asian (of Chinese descent). Baseline measurements will include measurement of coronary calcium using computed tomography; measurement of ventricular mass and function using cardiac magnetic resonance imaging; measurement of flow-mediated brachial artery endothelial vasodilation, carotid intimal-medial wall thickness, and distensibility of the carotid arteries using ultrasonography; measurement of peripheral vascular disease using ankle and brachial blood pressures; electrocardiography; and assessments of microalbuminuria, standard CVD risk factors, sociodemographic factors, life habits, and psychosocial factors. Blood samples will be assayed for putative biochemical risk factors and stored for use in nested case-control studies. DNA will be extracted and lymphocytes will be immortalized for genetic studies. Measurement of selected subclinical disease indicators and risk factors will be repeated for the study of progression over 7 years. Participants will be followed through 2008 for identification and characterization of CVD events, including acute myocardial infarction and other coronary heart disease, stroke, peripheral vascular disease, and congestive heart failure; therapeutic interventions for CVD; and mortality.

cardiovascular diseases; cardiovascular system; cohort studies; coronary disease; epidemiologic methods; prospective studies

Abbreviations: Abbreviations: CVD, cardiovascular disease; MESA, Multi-Ethnic Study of Atherosclerosis.


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